Name: Suiany Vitorino Gervásio
Type: MSc dissertation
Publication date: 22/02/2019
Advisor:
Name |
Role |
|---|---|
| Geralda Gillian Silva Sena | Co-advisor * |
| Maria do Carmo Pimentel Batitucci | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Geralda Gillian Silva Sena | Co advisor * |
| Hildegardo Seibert França | Internal Examiner * |
| Juliana Macedo Delarmelina | External Examiner * |
| Maria do Carmo Pimentel Batitucci | Advisor * |
| Ricardo Machado Kuster | External Alternate * |
| Silvia Tamie Matsumoto | Internal Alternate * |
Summary: Secondary metabolites constitute an important part of the plants. Present
protection function,assist in environmental responses and adaptation of plants
to the environment. Its chemical composition is what favors the activities
performed by secondary metabolites and enables its most diverse applications,
whether for the treatment of diseases with the direct use of plants or production
for industry. In search of new sources of secondary metabolites, the algae and
fungi presented themselves as prominent groups, being used because of how
ease is the production of these metabolites; without requiring large spaces and
investments. Among the fungi, the genus Botryospaheria, known to cause
diseases in plants, houses the species Botryosphaeriarhodina, which causes
eucalyptus cancer, a promoter of fruit rot and is known for the production
ofbotriosferana, a polysaccharide producedextracellularly, of type β-
glucana with type β-(1 → 3.1 → 6) links and of low solubility.
Botriosferanapresents antioxidant activity as well ashypocholesterolemic,
antimutagenic, antifungal and antiviral activity. Aiming to potentialize these
activities this molecule has undergone a structural alteration,
carboxymethylation, WHERE the number of branches is increased and therefore
also its solubility. To verify that the increase in solubility has altered its activity,
analyses of the antioxidants were performed by methods of DDPH, Abts, Frap
and Iron chelating2+as well as the allelopathic activityin the test systems Allium
Strain and Lactuca sativa, the cellular viability of human lymphocytes and
sarcoma 180 by the MTT methodand the evaluation of mutagenic activity and
antimutagenic in mice. The carboxymethylated botriosferana presented
antioxidant activity only in two methods, DPPH and iron chelating2+, not
presenting anyallelopathicactivity. Increased cell viability of lymphocytes, mainly
under 48h of exposure, and was selective to decrease the cell viability of 180
sarcoma under 48h of exposure. It did not present mutagenic activity, but was
antimutagenic, considerably decreasing the micronuclei values compared to
positive control. The results obtained indicate that the structural alteration
maintained the activities previously described and which were reassessed by
comparing the reduction of damage caused by cyclophosphamide, there is an
indication that the structural change potentiated this activity of the
carboxymethylatedbotriosferana. When considering all the activities performed
by botriosferana, it is possible to observe its potential application as an adjuvant
for the treatment of chronic non-communicable diseases, such as
hypercholesterolemia, and also for the treatment of cancer.
