Name: Plucia Franciane Ataide Rodrigues
Type: MSc dissertation
Publication date: 15/03/2023
Advisor:
Name |
Role |
|---|---|
| Hildegardo Seibert França | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Hildegardo Seibert França | Advisor * |
| JOSINEI RODRIGUES FILHO | External Examiner * |
| Viviana Borges Corte | Internal Examiner * |
Summary: Carapa guianensis Aubl and Copaifera reticulata Benth are considered one of the most
valuable species by Amazonian riverside dwellers for biological activities, such as analgesic,
antibacterial, anti-inflammatory, antifungal, antiallergic action, being effective against
wounds, bruises, rheumatism, ear infections and insect repellent. The present study aimed to
evaluate the chemical composition, the antioxidant capacity in vitro, the cytotoxicity of the
vegetable oils of andiroba and copaiba. The vegetable oils of C. guianensis and C. reticulata
were purchased from the Cooperative of Amazonian Products (CAMAIPI), in the
municipality of Mazagão-AP, lot 02/2020. The oil samples were prepared by successive
dilutions in concentrations of 75, 100, 150, 200, 250 and 300 mg/mL to perform the ABTS
and DPPH antioxidant tests. For total phenols, the concentrations were 60, 100, 200, 300
and 400 mg/mL. The cytotoxic activity was by MTT colorimetric assay using CHO-K1
epithelial cells. As for the exposure of Allium cepa seeds, the oil was diluted in 1% tween
80 at concentrations of 1, 5, 10 and 15 mg/mL. The analysis of cytotoxicity of andiroba oil
did not result in significant loss of mitochondrial function compared to the negative control,
since the treatments showed high percentage of viable cells ranging from 82 to 127% of cell
viability. However, copaiba oil was cytotoxic in all concentrations, with percentage of cell
viability ranging from 31 to 37.67% and inhibited the germination of seeds of A. cepa in
almost all concentrations. The C. guianensis oil presented IC50 of 254.2 ± 10 mg/mL and
274.9 ± 6.19 mg/mL for DPPH and ABTS, respectively and 80.0 ± 0.01 mg TE/ g -1 in the
evaluation of total phenols by the Folin-Ciocalteu reaction. In the phytochemical prospection
it could be observed only the presence of fatty acids with stain in the same Rf of the oleic
acid standard. The compounds identified by GC/MS were derivatives of fatty acids, such as
oleic acid, palmitic acid, stearic acid and linoleic acid with realtive area of 32.85; 26.06;
13.28; 11.79 %, respectively. In the antioxidant activity, C. reticulata obtained IC50 of 48.30
± 4.80 and 139.25 ± 1.86 mg/mL for ABTS and DPPH respectively. The evaluation of total
phenols was done by Folin-Ciocalteu reaction and it was obtained 50.05 ± 0.01 (mg trolox/g
-1). Through CCD were identified important metabolic groups, such as fatty acids,
coumarins, alkaloids and tannins. With this work we conclude that the andiroba vegetable
oil presents antioxidant activity in vitro, low cytotoxicity in epithelial CHO-K1 cells and
with the presence of oleic and palmitic acid representing more than 50% of the chemical
composition of the oil. As for the oil-resin of copaiba, despite having shown cytotoxicity, it
has good antioxidant activity and its biological properties may be important to combat cancer
cells and for application in the agricultural sector. It is suggested that the seeds are tested
again with discontinuous treatment using two indicators for better evaluation of
phytotoxicity and other parameters.
Key-words: Andiroba. copaiba. cytotoxicity. antioxidant. phenolic compounds. oil-resin.
