Name: MAINÃ MANTOVANELLI DA MOTA
Type: PhD thesis
Publication date: 28/02/2019
Advisor:
Name |
Role |
|---|---|
| Maria do Carmo Pimentel Batitucci | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| ANNY CAROLYNE DA LUZ GROLA | External Alternate * |
| Antelmo Ralph Falqueto | Internal Alternate * |
| Jose Aires Ventura (M/D) | Internal Examiner * |
| Maria do Carmo Pimentel Batitucci | Advisor * |
| Ricardo Machado Kuster | External Examiner * |
| Silvia Tamie Matsumoto | Internal Examiner * |
| Taís Cristina Bastos Soares | External Examiner * |
Summary: The medicinal plants have been used for treatment, cure and prevention of
diseases for several thousands of years. Varronia curassavica and Momordica
charantia are widely exploited plant species for therapeutic purposes. Varronia
curassavica Jacq. popularly known as erva-baleeira, belongs to the family
Cordiaceae and is traditionally used to treat inflammation, in addition it is
described by present antibacterial, antifungal, anti-allergic, antitumor, and
antioxidant properties. Already the Momordica charantia L. is a species
herbaceous belonging to the family Cucurbitaceae commonly known as bitter
gourd or bitter melon and in tradicional medicine, it is used for the treatment of
diabetes, colics, cancer, among other disorders. The medicinal properties
shown by different medicinal plants are due to the secondary metabolites
present in the plant. However, genetics and environmental factors, such as soil
composition, temperature, rainfall and ultraviolet radiation incidence can affect
the concentrations of these chemical components that reflect on their biological
activities. Therefore, the aim of this study was to evaluated the environmental
and genetic factors influence on the production of secondary metabolites and
the antioxidant, cytotoxic and antiproliferative activity of populations from
different regions of Espírito Santo/Brazil of V. curassavica and M. charantia.
The results obtained from the analyzes using ISSR molecular markers showed
that both V. curassavica and M. charantia species showed a significant
similarity between the analyzed populations, probably due to the fact these
species possess variety of pollinators insect and seed dispersal animals which
facilitated the gene flow. The phytochemical analyzes of V. curassavica
revealed a significant quantitative difference between the samples tested, which
reflected in variability in their biological antioxidant and antitumoral activities.
Results strongly suggest that these variations were caused by environmental
rather than genetic factors. The analyzes with the species M. charantia showed
there is not a very large variation among the populations related as to their
chemical content and biological activity, suggesting that for this plant the
geographic location is not determinant for the quantitative and qualitative
variation of phenolic compounds. The extract of both species showed a higher
selective cytotoxicity in vitro against sarcoma 180, demonstrating that V.
curassavica and M. charantia presents therapeutic potential for the
development of new drugs. This work are important to help in elucidation
optimal conditions for ethnopharmacological use of these medicinal plants.
Keywords: Varronia curassavica Momordica charantia ISSR phenolic
compounds DPPH ABTS FRAP antitumoral activity MTT
