Name: Fernanda Bonini dos Reis
Type: MSc dissertation
Publication date: 28/09/2021
Advisor:

Name	Role
Camilla Rozindo Dias Milanez	Advisor *

Examining board:

Name	Role
Camilla Rozindo Dias Milanez	Advisor *
Geraldo Rogério Faustini Cuzzuol	Internal Alternate *
HIULANA PEREIRA ARRIVABENE	Co advisor *
LEONARDO VALANDRO ZANETTI	External Examiner *
Silvia Tamie Matsumoto	Internal Examiner *
VINÍCIUS NOVO GAMA	External Alternate *

Summary: The acidity of soils composed mainly of aluminosilicate minerals is a natural
condition in tropical regions. Soils with a pH value close to 5.0 greatly affect
Al3+ availability, a phytotoxic form for some species. High concentrations of
Al3+ are found in the Floodable Forest, a restinga´s phytophysiognomy type
belonging the Atlantic tropical forest. Some plant species adapted to acidic soils
develop aluminum exclusion or tolerance mechanisms (accumulators). This
study aimed to investigate whether woody species occurring in the restinga
Floodable Forest of the Paulo César Vinha State Park, Guarapari - ES are
aluminum accumulators; quantify and verify the main sites of storage of this
element and describe the leaf and stem anatomy of the accumulating species.
For this purpose, the accumulating species were identified through
quantification of Al content in leaves and stems of 28 species belonging to 24
families. For the histolocalization of Al, cross sections of leaves (limb and
petiole) and stems (primary and secondary structure) of fresh material were
tested with chrome azurol S. The anatomical and histochemical characterization
of the accumulating species was performed according to techniques of plant
anatomy. The chemical analyzes revealed that the greatest aluminum
accumulation occurred preferentially in the leaves in relation to the stems.
Among the species evaluated, Miconia sp.1, Miconia sp.2 and Laplacea
fruticosa were identified as Al accumulators. Although Pouteria cuspidata did
not show Al content up to 1000 mg/kg of leaf dry mass, this species showed
high content of Al in latex (4,991 mg/kg), that´s why we included this species in
the other accumulators. Histochemical analysis showed that Al was found
predominantly impregnating cell walls, and may also accumulate in the content
of some cells. The presence of Al in lignified cells was not very common, but in
P. cuspidata and Miconia sp.1 was observed occurred accumulation in vessel
elements in the process of differentiation and in the inner wall of gelatinous
fibers, respectively. It is concluded that the four Al-accumulating woody species
do not follow a pattern of compartmentalization in leaves and stems, not even
for species of the same genus. In Miconia, aluminum occurs in the same sites
of accumulation of phenolic compounds, and deserve further studies in order to
understand the relationship between these two elements.
Kay words: Aluminotoxic, stem, leaf, histochemistry, morphology.