Name: Rodolpho Henrique Waichert da Silva
Type: PhD thesis
Publication date: 24/02/2022
Advisor:
Name |
Role |
|---|---|
| Alessandro Coutinho Ramos | Co-advisor * |
| Viviana Borges Corte | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Alessandro Coutinho Ramos | Co advisor * |
| Frederico Jacob Eutrópio | External Examiner * |
| Hildegardo Seibert França | Internal Examiner * |
| JEAN CARLOS VENCIONECK DUTRA | External Examiner * |
| JOSINEI RODRIGUES FILHO | External Alternate * |
| Pedro Corrêa Damasceno Junior | Internal Examiner * |
| Valéria de Oliveira Fernandes | Internal Alternate * |
| Viviana Borges Corte | Advisor * |
Summary: Understanding the toxic effects of iron on plant physiological processes appears as an
important tool for promoting actions that aim to enhance agricultural production in areas
under the influence of mining activities or whose environmental characteristics favor the
bioavailability of the metal. The objective of this work is to verify the physiological
alterations in the germination and growth of common bean (P. vulgaris) and mayze (Z.
mays) submitted to planting under high iron conditions. The plants were submitted to
concentrations of 1, 4, and 8 mM of iron in the forms of Fe-EDTA, Ferrous Sulfate
(Fe+2), and P. vulgaris underwent complementary treatment with Iron Chloride (Fe+3) at
the same concentrations as the other sources. The seeds were germinated in a
germination chamber B.O.D., using a constant temperature of 25 ºC and a photoperiod
of 12h/12h. The analyzed variables were the germination percentage (%G), germination
speed index. Plants were grown in 0.5L polyethylene pots in an inert substrate, fed
Hoagland`s solution at half ionic strength and pH 5.0 and the following variables were
analyzed: Leaf area, stem length, root length, dry mass, quantification of chloroplast
pigments, analysis of chlorophyll fluorescence. The activity of the antioxidant enzymes
SOD, CAT, POX was verified for the young seedlings. Iron stress did not change the
germination of the evaluated species, however it affected the initial growth and biomass
allocation at concentrations of 4 mM and 8 mM for the different sources of Fe. The
species P. vulgaris did not show variations in the levels of chloroplast pigments.
between treatments, however, in Z. mays the pigments varied in relation to the control
in all treatments. The pollutant did not affect the integrity of the photosynthetic process
of the evaluated species. The action of antioxidant enzymes was not observed in P.
vulgaris, and the use of non-enzymatic routes to combat ROS is characteristic of the
species, WHEREas Z. mays showed POX action in the Fe+2 treatment. Thus, it can be
concluded that Fe concentrations above 4 mM generate changes in the growth of the
evaluated cultivars.
