Name: ANNY CAROLYNE DA LUZ GROLA
Type: PhD thesis
Publication date: 22/03/2018
Advisor:
Name |
Role |
|---|---|
| Maria do Carmo Pimentel Batitucci | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Antelmo Ralph Falqueto | Internal Alternate * |
| Elias Terra Werner | Internal Examiner * |
| Jose Aires Ventura (M/D) | Internal Examiner * |
| Maria do Carmo Pimentel Batitucci | Advisor * |
| Rita de Cássia Ribeiro Gonçalves | External Examiner * |
| Rodrigo Rezende Kitagawa | External Alternate * |
| Sarah Maria Vargas | External Examiner * |
Summary: ABSTRACT
Bidens pilosa and Tithonia diversifolia are plants from Asteraceae family with
distribution in tropical and subtropical regions. They are used for several therapeutic
purposes: anti-inflammatory, antiseptic, hepratoprotective, chemopreventive, among
others. The plants of this family have a complex chemical defense system, with
secondary metabolites derived: phenolic compounds, flavonoids, lactones
serquiterpenes and polyacetylenes. These compounds have their production and
accumulation modified in response to environmental, ontogenetic and also hereditary
variations. Thus, it is important to control and standardize the steps involved in the
production of phytopharmaceuticals, from the selection of the initial plant material, the
conditions of cultivation, post-harvest management and methods of extraction of the
bioactive compounds. The objectives of this study were to evaluate RAPD genetic
markers of different populations of B. pilosa and T. diversifolia. And, also, to analyze
how the management of the culture conditions can influence the growth, the
photosynthetic parameters, the production of secondary metabolites, the antioxidant
and cytotoxic activity of these plants. B. pilosa is a species with several
characteristics that guarantee a high genetic variability, nevertheless presented low
levels of similarity, indicating that the grouping found is not only related to the
geographical proximity,though probably to the dispersion of seeds through animals
and the contamination of species cultivated with B. pilosa seeds, which facilitated the
gene flow. On the other hand, for T. diversifolia high levels of genetic diversity were
found, not positively correlated with the proximity of geographic locations. Although
often propagated by cuttings, T. diversifolia reproduces sexually, with large numbers
of seeds and abundant variety of insect pollinators, characteristics that contribute to
greater genetic variability. Regarding the management of the cultivation conditions, in
general, the fertilizer applications increased the photosynthesis and the total dry
mass in the two species studied. However, the plants that did not receive fertilization
presented the highest levels of phenolic compounds and higher antioxidant activity, a
result related to the lower availability of nitrogen. These results indicate that there is
an increase in the production of secondary metabolites when liquid photosynthesis
decreases, leading to an up-regulation of shikimic acid in the pentose phosphate
pathway. The species did not presents cytotoxic activity in human lymphocytes, while
reducing cell viability in sarcoma 180. It is important to standardize the management
cultivation of Asteraceae, in order to achieve high levels of phenolic compounds and
biomass.
Keywords: RAPD Phenolic Coumponds Antoxidant Activity Bidens pilosa
Tithonia diversifolia Photosyntesis HPLC MTT Mineral nutrition water déficit
