Name: MONIQUE ELLEN FARIAS BARCELOS
Type: PhD thesis
Publication date: 19/02/2019
Advisor:
Name |
Role |
|---|---|
| Maria do Carmo Pimentel Batitucci | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| Antelmo Ralph Falqueto | Internal Alternate * |
| Elias Terra Werner | Internal Examiner * |
| Geralda Gillian Silva Sena | External Examiner * |
| Hildegardo Seibert França | Internal Examiner * |
| Juliana Macedo Delarmelina | External Examiner * |
| Maria do Carmo Pimentel Batitucci | Advisor * |
| Ricardo Machado Kuster | External Alternate * |
Summary: ABSTRACT
Mikania glomerata Spreng is a very studied species on the ethnopharmacological
aspect and production of secondary compounds, such as coumarins. These
compounds have been associated with the use of nanoparticles which are used to
associate plant-active compounds, that are poorly soluble, increasing the
bioavailability of these. Thus, the present study aims to evaluate the ionic capacity of
the extract and nanodispersion of the content of coumarin (0.001; 0.002; 0.004; 0.008
and 0.016 mg/mL) of Mikania, relating this ability with a potential allelopathic and
mutagenic activity in Roots of Lactuca sativa and Allium Cepa. The H + flow was
determined in the root elongation zone using selective ion vibratory system.
Allelopathic activity was evaluated by the germination test. After its protrusion, the
roots were photographed and analyzed in ImageJ software. To evaluate the mutagenic
activity, the seeds were germinated on Petri dishes in triplicate and received 5mL of
Mikania extract in the same concentrations used for nanodispersion, in addition to the
groups: pure Coumarin 0.002 mg/mL (CuP); Positive control methylmethosulfonate at
4x10-4M (MMS)-as positive control-and negative control (distilled water). After root
protrusion, slides were made for analysis of the mitotic index and possible
chromosomal abnormalities. The data obtained were submitted to ANOVA and test of
averages (p < 0.05). The flow of H +, in the dose-response curve, indicated the
concentration of 0.002 mg/mL, being the best concentration for comparison to the
other treatments. It was also possible to observe that all treatments promoted inhibition
of the H + flow when compared to the negative control. Similarly, it was found in the
flow of coumarin content in nanodispersion, in which it was possible to evidence an
oscillation, according to the concentrations analyzed. This result may be related to the
inhibition of seed germination, abnormal cell differentiation and reduction of plant
growth. In the analysis of allelopathic activity, it was observed that Lactuca sativa
showed a higher decrease in the percentage of germination and a more expressive
increase in the index of Allelpathy, when compared to Allium Cepa, at the
concentration of 0, 004mg/mL of coumarin content in the extract. However, for the test
with nanodispersion, it was evidenced that, in both testing organisms, there was a
significant drop in germination and/or inhibition of germination, when compared to
control, providing greater allelopathic activity. As for the mutagenic activity, in
continuous treatment, the CuP promoted a greater clastogenic effect, indicating that
the isolated coumarin promotes more damage to the plant cell than when associated
with other compounds present in the Guaco extract. In the discontinuous treatment of
the extract, it was possible to verify that, for 24h, 48h, and for the treatment of recovery,
there was a decrease in the mitotic index. In the 24h treatment, the concentration of
0, 001mg/mL had a clastogenic effect when compared to the negative control.
Keywords: Protons Cytotoxicity Allelopathic Allium cepa.
